Abstract:
Multiphoton microscopy is an indispensable tool for high-resolution, noninvasive imaging of structure and function deep within intact tissues and organs. In this talk, we focus on recent advances in technology development that enable in vivo imaging at unprecedented depths in mouse brain, lymph node, and cancer models. We will discuss the fundamental depth limits of high–spatial resolution optical imaging in scattering biological tissues, and describe new approaches that allow imaging of mouse brain structure and function at depths of 2 mm and beyond. We will also present several directions for continued technology development aimed at imaging deep, wide, and fast, including advances in laser sources and the design of optimal illumination schemes. Finally, we will briefly discuss applications of deep multiphoton imaging beyond the brain, highlighting emerging opportunities in immunology and cancer biology.